- RUNX2: Runt related transcription factor 2
- Beta-catenin: β-catenin in postnatal Osx-lineage cells critically regulates bone homeostasis by promoting osteoblast activity and suppressing osteoblast turnover, while restraining osteoclast and marrow fat formation
- Osterix: Regulates calcification and degradation of chondrogenic matrices
- BMPs: Bone morphogenic proteins
- Wnt: Wnt signaling has been shown as an important regulatory pathway in the osteogenic differentiation of mesenchymal stem cells
- PTH: Parathyroid hormone
- IGF: Insulin-like growth factor
Why PRF Does Not Workfor Bone Regeneration:Literature Review
Sheet of osteoblastic cells combined with platelet-rich fibrin improves the formation of bone in critical-size calvarial defects in rabbits.
Wang Z, Hu H, Li Z, Weng Y, Dai T, Zong C, Liu Y, Liu B.
Br J Oral Maxillofac Surg. 2016 Apr;54(3):316-21. doi: 10.1016/j.bjoms.2015.12.015. Epub 2016 Jan 15.
Techniques that use sheets of cells have been successfully used in various types of tissue regeneration, and platelet-rich fibrin (PRF) can be used as a source of growth factors to promote angiogenesis. We have investigated the effects of the combination of PRF and sheets of mesenchymal stem cells (MSC) from bone marrow on the restoration of bone in critical-size calvarial defects in rabbits to find out whether the combination promotes bony healing. Sheets of MSC and PRF were prepared from the same donor. We then implanted the combined MSC and PRF in critical-size calvarial defects in rabbits and assessed bony restoration by microcomputed tomography (microCT) and histological analysis. The results showed that PRF significantly increased bony regeneration at 8 weeks after implantation of sheets of MSC and PRF compared with sheets of MSC alone (p=0.0048). Our results indicate that the combination of sheets of MSC and PRF increases bone regeneration in critical-size calvarial defects in rabbits, and provides a new way to improve skeletal healing.
In this animal study, the authors found that there was a benefit to adding PRF to sheets of mesenchymal stem cells.
Leukocyte-platelet-rich plasma (L-PRP) induces an abnormal histophenotype in craniofacial bone repair associated with changes in the immunopositivity of the hematopoietic clusters of differentiation, osteoproteins, and TGF-β1.
Giovanini AF1, Grossi JR, Gonzaga CC, Zielak JC, Göhringer I, Vieira Jde S, Kuczera J, de Oliveira Filho MA, Deliberador TM.
Clin Implant Dent Relat Res. 2014 Apr;16(2):259-72. doi: 10.1111/j.1708-8208.2012.00478.x. Epub 2012 Jul 16.
Unlike autograft and sham groups, the defects treated with L-PRP demonstrated significant positivity to TGF-β1, while the BMP2 was scarce. These results coincided with the lower bone matrix deposited and larger medullary area, which were composed of fibrosis, when treated with only L-PRP, or intense adiposity on defects filled with L-PRP mixed with autograft. The fibrosis that occurred was associated with a minor percentage of osteoproteins, intense presence of CD34(+) CD45(-) cells, and significant expression of TGF-β1 in all time periods analyzed. The adiposity occurred from the major presence of osteoprogenitor BMPR1B (+) Runx2(+) cells simultaneously to BMP2(-) TGF-β1(+) and CD34(+) CD45(+/-) expressions predominantly on the earlier period.
From this study, it can be concluded that the L-PRP used alone or mixed to autograft hindered the osteoneogenesis due to suppression of immunoexpression of BMP2, while the immunopositivity of TGF-β1 was intense. When used alone, the L-PRP induced a fibrotic condition associated with TGF-β1 presence and lack of osteoproteins, but when L-PRP was mixed to autograft, it induced the presence of the osteolineage cells (BMPR1B (+) Runx2(+) ), but also inhibited the terminal osteoblastic maturation associated with the lack of BMP2 and the presence of TGF-β1(+), a fact that contributed to cellular transdifferentiation into fat cells.
This animal study found that L-PRF inhibited bone formation and resulted in fat formation when mixed with an autograft.
Effects of leukocyte-platelet rich fibrin on postoperative complications of direct sinus lifting.
Gurler G1, Delilbasi C.
Minerva Stomatol. 2016 Aug;65(4):207-12.
Pain, swelling, sleeping, eating, phonetics, activities of daily living, missed work days and soft tissue healing were evaluated postoperatively.
Data of 24 patients were evaluated. Improvements were seen in the studied parameters in the L-PRF group; however, the difference was not significant between the two groups (P>0.05).
The use of L-PRF and allogenous bone graft in combination with L-PRF membrane does not significantly improve postoperative complications following direct sinus lifting.
L-PRF does not improve post-operative healing in humans.
Effect of leukocyte- and platelet-rich fibrin (L-PRF) on bone regeneration: a study in rabbits.
Knapen M1, Gheldof D, Drion P, Layrolle P, Rompen E, Lambert F.
Clin Implant Dent Relat Res. 2015 Jan;17 Suppl 1:e143-52. doi: 10.1111/cid.12146. Epub 2013 Sep 4.
Materials and Methods
A total of 72 hemispheres were implanted on the calvaria of 18 rabbits and filled with three different space fillers: L-PRF, bovine hydroxyapatite (BHA), BHA + L-PRF, and an empty hemisphere was used as control. Six rabbits were sacrificed at three distinct time points: 1 week, 5 weeks, and 12 weeks. Histological and histomorphometrical analyses were carried out.
No statistical differences were found within the four groups in terms of bone quantity and quality at each timepoint (p = .3623).
According to the present study, L-PRF does not seem to provide any additional effect on the kinetics, quality, and quantity of bone in the present model of guided bone regeneration.
In this rabbit study, L-PRF did not improve healing over no graft or when mixed with bone graft materials.
The Effect of Concentrated Growth Factor on Rat Bone Marrow Cells In Vitro and on Calvarial Bone Healing In Vivo.
Takeda Y, Katsutoshi K, Matsuzaka K, Inoue T.
Int J Oral Maxillofac Implants. 2015 Sep-Oct;30(5):1187-96. doi: 10.11607/jomi.3995.
Cell proliferation and osteoblastic differentiation were significantly higher in cells cultured on the CGF-coated disks than on the PPP gel-coated disks. In vivo, more new bone had formed in defects treated with CGF than in defects treated with PPP gel.
In this preliminary study, fibrin and soluble factors in CGF promoted initial cell stretching, proliferation, and osteoblastic differentiation of RBM cells in vitro and bone regeneration in rat calvarial bone defects in vivo.
In this animal study, the authors found that CGF improved bone healing in rabbits.
Comparison of removal torques of SLActive® implant and blasted, laser-treated titanium implant in rabbit tibia bone healed with concentrated growth factor application.
Park SH, Park KS, Cho SA.
J Adv Prosthodont. 2016 Apr;8(2):110-5. doi: 10.4047/jap.2016.8.2.110. Epub 2016 Apr 21.
It was found that BLT surface modification exhibited excellent osseointegration. In addition, CGF application did not affect the insertion and removal torque of the implants.
CGF produced no clinical benefit to implant integration.
Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing.
Kim TH, Kim SH, Sándor GK, Kim YD.
Arch Oral Biol. 2014 May;59(5):550-8. doi: 10.1016/j.archoralbio.2014.02.004. Epub 2014 Feb 15.
In micro-CT analysis, bone mineral density and bone volume were greater in the experimental group than in controls at both 6th and 12th week, but not among the experimental groups. The histomorphometric examination showed no significant difference between the bone formation at 12 weeks among control or experimental groups.
PRP, CGF and PRF showed improved bone formation over no graft when evaluated using Micro CT scan. However, histologically there was no difference between PRP, CGF, PRF and no graft. There was no statistic difference in the bone formed by PRP, PRF and CGF.
Comparative release of growth factors from PRP, PRF, and advanced-PRF.
Kobayashi E, Flückiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ.
Clin Oral Investig. 2016 Dec;20(9):2353-2360. doi: 10.1007/s00784-016-1719-1. Epub 2016 Jan 25.
The results from the present study indicate that the various platelet concentrates have quite different release kinetics. The advantage of PRP is the release of significantly higher proteins at earlier time points whereas PRF displayed a continual and steady release of growth factors over a 10-day period. Furthermore, in general, it was observed that the new formulation of PRF (A-PRF) released significantly higher total quantities of growth factors when compared to traditional PRF.
The growth factors released from the palette preparations were PDGF-AA followed by PDGF-BB, TGFB1, VEGF, and PDGF-AB. None of these growth factors promote bone growth.
Early Bone Formation at a Femur Defect Using CGF and PRF Grafts in Adult Dogs: A Comparative Study.
Park HC, Kim SG, Oh JS, You JS, Kim JS, Lim SC, Jeong MA, Kim JS, Jung C, Kwon YS, Ji H.
Implant Dent. 2016 Jun;25(3):387-93. doi: 10.1097/ID.0000000000000423.
At 4 weeks, the comparisons of each experimental group showed a significant difference between the CGF group and the synthetic bone graft group. When comparing the CGF and allograft material groups, the allograft group showed significantly more new bone formation. In the case of vascular endothelial growth factor, CGF had 1.5 times more than PRF. CGF showed a fibrinogen structure with a constant diameter.
When applied to a clinical case, CGF is predicted to show better results than PRF.
As is the case with some articles published in Implant Dentistry, it appears there is little editing or peer review. The results and conclusions make no sense so, we will summarize the findings:
- They evaluated the concentration of growth factors and they could not find TGF-B in either PRF or CGF.
- They did find VEGF, but there was no difference in concentration between PRF and CGF.
- The amount of new bone formation and the amount of bone in contact with the implant was significantly higher in the synthetic bone graft group than the control, PRF, or CGF groups.
Bone tissue engineering with bone marrow-derived stromal cells integrated with concentrated growth factor in Rattus norvegicus calvaria defect model.
Honda H1, Tamai N, Naka N, Yoshikawa H, Myoui A.
J Artif Organs. 2013 Sep;16(3):305-15. doi: 10.1007/s10047-013-0711-7. Epub 2013 May 23.
In the in-vivo study, the CGF group regenerated bone better than the control group, and combined therapy with CGF and BMSCs almost completely repaired critical-size bone defects within 12 weeks after surgery. In the in-vitro study, the CGF extract, at concentrations between 1 and 10%, promoted proliferation, osteogenic maturation, and mineralization of hTERT-E6/E7 human MSCs in a dose-dependent manner but had an inhibitory effect at higher concentrations. In conclusion, a CGF extract promoted the proliferation, osteogenic maturation, and mineralization of mesenchymal stem cells in vitro, and combination therapy with CGF and BMSCs resulted in excellent healing of critical-size bone defects in vivo.
Whenever we see words like “excellent” used in an abstract, we know the authors are trying to sell something and that is the case with this study. This is the first study we have reviewed that did a quantitative real-time PCR analysis on mesenchymal stem cells to evaluate which genes are activated by the platelet rich preparations. In this case, CGF was added to stem cells and the authors found that CGF had no effect on the genes that are involved in osteogenesis. CGF had no effect on the expression of RUNX2 or osterix, which are the master regulators of osteogenesis. If they are not increased, they will likely not stimulate bone formation.
PDGF-regulated miRNA-138 inhibits the osteogenic differentiation of mesenchymal stem cells.
Qu B, Xia X, Wu HH, Tu CQ, Pan XM.
Biochem Biophys Res Commun. 2014 Jun 6;448(3):241-7. doi: 10.1016/j.bbrc.2014.04.091. Epub 2014 May 2.
Differentiation-specific microRNAs may play a critical role in MSC differentiation, and they can be altered by PDGF signaling. We propose that PDGF modulates MSC differentiation by regulating microRNA expression. Therefore, we investigated whether PDGF treatment could alter the expression profile of miRNAs in MSCs. Furthermore, we assessed the osteoblast phenotype of MSCs after inducing osteogenic differentiation. We found that PDGF treatment significantly inhibits the osteogenic differentiation of MSCs and that miR-138 gene transcription is controlled by PDGF signaling. Our results confirm that miR-138 inhibits the osteogenic differentiation of MSCs and suppresses the phosphorylation of FAK, ERK1/2, and Runx2. Furthermore, our study clearly demonstrates that downregulation of Runx2 by miR-138 is critical for the PDGF-mediated inhibition of osteogenic differentiation of MSCs.
PDGF is a main growth factor in platelet preparations. This study clearly outlines how this molecule inhibits bone formation.
Tumor Necrosis Factor-α (TNF-a) Attenuates the Osteogenic Differentiation Capacity of Periodontal Ligament Stem Cells by Activating Protein Kinase Like Endoplasmic Reticulum Kinase (PERK) Signaling.
Tan J, Zhou L, Xue P, An Y, Luo L, Zhang R, Wu G, Wang Y, Zhu H, Wang Q.
J Periodontol. 2016 Aug;87(8):e159-71. doi: 10.1902/jop.2016.150718. Epub 2016 Apr 18.
Higher concentrations of TNF-α (10 and 20 ng/mL) impaired osteogenic differentiation of PDLSCs but activated the PERK pathway. Pretreatment of PDLSCs with lower concentrations of 4-PBA prevented the TNF-α-induced upregulation of GRP78, PERK, and ATF4 and recovered differentiation ability. Finally, PERK knockdown also restored osteogenic differentiation.
TNF-α attenuates osteogenic differentiation ability of PDLSCs through activation of the PERK pathway.
TNF is another significant growth factor in platelet preparations and this study shows how it inhibits bone formation.
- In approximately 50% of the animal studies, platelets preparations showed improved bone growth
- In approximately 50% of the animal studies, the platelet preparations showed no improvement in bone growth
- Platelet preparations do not contain bone growth factors and do not stimulate the production of bone growth factors
- There were no human studies that showed any benefit for bone growth when the preparations were mixed with bone grafts
- There were no human studies that showed any benefit for bone growth when the platelet preparations were used alone